Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars
Identifieur interne : 000C74 ( Main/Exploration ); précédent : 000C73; suivant : 000C75Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars
Auteurs : Evans Nyaboga [Kenya, Suisse] ; Joshua Njiru [Kenya] ; Edward Nguu [Kenya] ; Wilhelm Gruissem [Suisse] ; Herve Vanderschuren [Suisse] ; Leena Tripathi [Kenya]Source :
- Frontiers in Plant Science [ 1664-462X ] ; 2013.
Abstract
Cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the USA, Europe and China; and its implementation and maintenance in African laboratories has remained scarce. The impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in Africa, where cassava has an important socioeconomic niche. A major constraint to the development of genetic transformation technologies for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite the success achieved in genetic modification of few cassava cultivars, including the model cultivar 60444, transgenic cassava production remains difficult for farmer-preferred cultivars. In this study, a protocol for cultivar 60444 developed at ETH Zurich was successfully implemented and optimized to establish transformation of farmer-preferred cassava cultivars popular in east Africa. The conditions for production and proliferation of friable embryogenic calli (FEC) and
Url:
DOI: 10.3389/fpls.2013.00526
PubMed: 24400011
PubMed Central: 3872047
Affiliations:
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Biochemistry, University of Nairobi</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Njiru, Joshua" sort="Njiru, Joshua" uniqKey="Njiru J" first="Joshua" last="Njiru">Joshua Njiru</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>International Institute of Tropical Agriculture</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Nguu, Edward" sort="Nguu, Edward" uniqKey="Nguu E" first="Edward" last="Nguu">Edward Nguu</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Department of Biochemistry, University of Nairobi</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Gruissem, Wilhelm" sort="Gruissem, Wilhelm" uniqKey="Gruissem W" first="Wilhelm" last="Gruissem">Wilhelm Gruissem</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule</institution><country>Zurich, Switzerland</country></nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Vanderschuren, Herve" sort="Vanderschuren, Herve" uniqKey="Vanderschuren H" first="Herve" last="Vanderschuren">Herve Vanderschuren</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule</institution><country>Zurich, Switzerland</country></nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Tripathi, Leena" sort="Tripathi, Leena" uniqKey="Tripathi L" first="Leena" last="Tripathi">Leena Tripathi</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>International Institute of Tropical Agriculture</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24400011</idno><idno type="pmc">3872047</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872047</idno><idno type="RBID">PMC:3872047</idno><idno type="doi">10.3389/fpls.2013.00526</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000F21</idno><idno type="wicri:Area/Pmc/Curation">000F20</idno><idno type="wicri:Area/Pmc/Checkpoint">000948</idno><idno type="wicri:Area/Ncbi/Merge">001550</idno><idno type="wicri:Area/Ncbi/Curation">001550</idno><idno type="wicri:Area/Ncbi/Checkpoint">001550</idno><idno type="wicri:Area/Main/Merge">000C77</idno><idno type="wicri:Area/Main/Curation">000C74</idno><idno type="wicri:Area/Main/Exploration">000C74</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars</title><author><name sortKey="Nyaboga, Evans" sort="Nyaboga, Evans" uniqKey="Nyaboga E" first="Evans" last="Nyaboga">Evans Nyaboga</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>International Institute of Tropical Agriculture</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule</institution><country>Zurich, Switzerland</country></nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea></wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Department of Biochemistry, University of Nairobi</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Njiru, Joshua" sort="Njiru, Joshua" uniqKey="Njiru J" first="Joshua" last="Njiru">Joshua Njiru</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>International Institute of Tropical Agriculture</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Nguu, Edward" sort="Nguu, Edward" uniqKey="Nguu E" first="Edward" last="Nguu">Edward Nguu</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Department of Biochemistry, University of Nairobi</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Gruissem, Wilhelm" sort="Gruissem, Wilhelm" uniqKey="Gruissem W" first="Wilhelm" last="Gruissem">Wilhelm Gruissem</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule</institution><country>Zurich, Switzerland</country></nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Vanderschuren, Herve" sort="Vanderschuren, Herve" uniqKey="Vanderschuren H" first="Herve" last="Vanderschuren">Herve Vanderschuren</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Biology, Plant Biotechnology, Eidgenössische Technische Hochschule</institution><country>Zurich, Switzerland</country></nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea></wicri:regionArea></affiliation></author><author><name sortKey="Tripathi, Leena" sort="Tripathi, Leena" uniqKey="Tripathi L" first="Leena" last="Tripathi">Leena Tripathi</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>International Institute of Tropical Agriculture</institution><country>Nairobi, Kenya</country></nlm:aff><country xml:lang="fr">Kenya</country><wicri:regionArea></wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Plant Science</title><idno type="eISSN">1664-462X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the USA, Europe and China; and its implementation and maintenance in African laboratories has remained scarce. The impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in Africa, where cassava has an important socioeconomic niche. A major constraint to the development of genetic transformation technologies for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite the success achieved in genetic modification of few cassava cultivars, including the model cultivar 60444, transgenic cassava production remains difficult for farmer-preferred cultivars. In this study, a protocol for cultivar 60444 developed at ETH Zurich was successfully implemented and optimized to establish transformation of farmer-preferred cassava cultivars popular in east Africa. The conditions for production and proliferation of friable embryogenic calli (FEC) and <italic>Agrobacterium-mediated</italic> transformation were optimized for three east African farmer-preferred cultivars (Ebwanatereka, Kibandameno and Serere). Our results demonstrated transformation efficiencies of about 14–22 independent transgenic lines per 100 mg of FEC for farmer-preferred cultivars in comparison to 28 lines per 100 mg of the model cultivar 60444. The presence, integration and expression of the transgenes were confirmed by PCR, Southern blot analysis and histochemical GUS assay. This study reports the establishment of a cassava transformation platform at International Institute of Tropical Agriculture (IITA) hosted by Biosciences eastern and central Africa (BecA) hub in Kenya and provides the basis for transferring important traits such as virus resistance and prolonged shelf-life to farmer-preferred cultivars in east Africa. 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Sayre</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Kenya</li><li>Suisse</li></country></list><tree><country name="Kenya"><noRegion><name sortKey="Nyaboga, Evans" sort="Nyaboga, Evans" uniqKey="Nyaboga E" first="Evans" last="Nyaboga">Evans Nyaboga</name></noRegion><name sortKey="Nguu, Edward" sort="Nguu, Edward" uniqKey="Nguu E" first="Edward" last="Nguu">Edward Nguu</name><name sortKey="Njiru, Joshua" sort="Njiru, Joshua" uniqKey="Njiru J" first="Joshua" last="Njiru">Joshua Njiru</name><name sortKey="Nyaboga, Evans" sort="Nyaboga, Evans" uniqKey="Nyaboga E" first="Evans" last="Nyaboga">Evans Nyaboga</name><name sortKey="Tripathi, Leena" sort="Tripathi, Leena" uniqKey="Tripathi L" first="Leena" last="Tripathi">Leena Tripathi</name></country><country name="Suisse"><noRegion><name sortKey="Nyaboga, Evans" sort="Nyaboga, Evans" uniqKey="Nyaboga E" first="Evans" last="Nyaboga">Evans Nyaboga</name></noRegion><name sortKey="Gruissem, Wilhelm" sort="Gruissem, Wilhelm" uniqKey="Gruissem W" first="Wilhelm" last="Gruissem">Wilhelm Gruissem</name><name sortKey="Vanderschuren, Herve" sort="Vanderschuren, Herve" uniqKey="Vanderschuren H" first="Herve" last="Vanderschuren">Herve Vanderschuren</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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